EFTA Document EFTA01929004

CLINICAL COMMUNICATION TO THE EDITOR First in Man: Adipose-derived Stromal Vascular Fraction Cells May Promote Restorative Cardiac Function the Mawr: Despite advances in clinical intervention, heart disease remains a causation of mortality in the world. Research demonstrates that the adipose-derived stromal vascular fraction of cells contains multi-potent cells that exhibit mesodermal and ectodermal capacity and contain CD44', CD90*, CD73*, CD105*. CD34*, and CD31* cells, which act in tissue regeneration and vascular stabilization.' We discuss a patient who underwent such cell administration as rescue therapy for failing cardiac function, despite conven- tional interventions. CASE SUMMARY The patient is a 73-year-old white man with a history of hyperlipidemia who presented with recurrent angina pecto- ris, for which he had undergone multiple angioplasties and 9 coronary stents (right posterior descending and distal right coronary beyond a saphenous vein graft to right coronary artery). He had further percutaneous coronary intervention to the distal right coronary artery and proximal posterior descending branch. His prior coronary bypass artery graft of 3 vessels had been redone. Robotic-assisted transmyocardial revascularization for angina was performed in the patient 2 months before cell delivery. He had significant left ven- tricular systolic dysfunction (ejection fraction, 20%) with a thinned. akinetic inferolateral segment. To evaluate left ventricular function, transesophageal echocardiography was performed in the patient. As a last resort intervention for the patient's dysfunc- tional heart. 100 million adipose-derived stromal vascular fraction cells were delivered via localized intramyocardial injection (left apex). and 200 million adipose-derived stro- mal vascular fraction cells were delivered systemically via intravenous injection. The intracoronary delivery of freshly isolated autologous cells consisted of a complex cell Funding: Provided by the University of Arizona Colkge of Medicine. Department of Surgery. Division of Cardiothoracie Surgery. Conflict of Interest: None. Authorship: All authors had access to the data and played a role in writing this manuscript. Requests for reprints should he addressed to Courtney Hemphill. BS. 1656 East Mabel St.. Tucson. AZ 85724. E-mail address: C.ibri,e,O'surg,.ry aritona cdu 00B2-9343/$ -see front matter O 2014 Elsevier Inc. All rights reserved. THE AMERICAN JOURNAL of MFDICINF composition. This uncultured population taken from lip. °aspirate, which had an approximately 86% viability, con- sisted of CD34' cells. Within 7 days of the procedure, transesophageal echo- cardiography was performed to evaluate recovery of the patient's left ventricular function. His ejection fraction improved to 35% with recovery in the contractility of the previously akinetic inferolateral segment. This confirmed the effectiveness of cell delivery in improving regional and global left ventricular systolic function. DISCUSSION Spontaneous recovery of the damaged myocardium occurred. The effective delivery of the stromal vascular fraction cell population. which contained stem cells, may have improved regional and global left ventricular systolic function, aiding recovery. Adipose-derived stromal vascular fraction cells may have cardiomyogenic potential. Cellular composition of cells administered included endo- thelial progenitor cells, vascular smooth muscle cells. mesenchymal stem cells. fibroblasts, growth factors, and pericytes. This complex population has shown efficacy in repairing damaged tissue to partial restoration of functional normalcy. Angiogenesis may contribute to restoration of cardiac function through preservation of remaining viable and hi- bernating cardiomyocytes. This salvaging effect might be maintenance of border zone cells through stem cell—mediated anti-apoptotic and neoangiogenic effects, which are causative of stem cell—produced paracrine factors.' This cell popula- tion has a high expression in DKK-I and R3 proteins, which are associated with regulation of stem cell proliferation and differentiation; CONCLUSIONS We postulate that the intramyocardial administration of multi-potent adipose-derived stromal vascular fraction cells and their diverse composition guided engraftment in the damaged area, facilitated the cardiomyocyte regenerative properties, and helped improve cardiac function. Because subcutaneous adipose tissue is readily available in most patients, adipose-derived stromal vascular fraction cells present a feasible clinical intervention for cardiac dysfunc- tion. The population complexity, which includes stromal vascular fraction and growth factors, likely promotes repair by recruiting cardiomyocytes. The autologous nature and multi-potency of these cells may contribute to them being ideal for cardiac function restoration. EFTA_R1_00377783 EFTA01929004 e12 The American Journal of Medicine, Vol 127, No 5, May 2014 Thin Khalpcy. MD, PhD'h" Rajesh Janardhanan, MDa-d John Konhilas, PhDI' Courtney Hemphill. BS' 'Division of Cardiothoracic Surgery Department of Surgery University of Arizona College of Medicine Tucson frDepartment of Physiology University of Arizona College of Medicine Tucson 'Deportment of Biomedical Engineering University of Arizona College of Medicine Tucson 'Department of Medicine and Medical Imaging University of Arizona College of Medicine Tucson lutp://dx.doi.org./10.1016/j.amjined.2013.12.024 ACKNOWLEDGMENTS The authors thank Or Steven Victor and Stephanie Squicciarini of IntelliCell Biosciences Inc (New York, NY) for supplying stromal vascular fraction, Dr Craig Hurst for providing lipoaspirate, and the patient and family. References I. Alt IL Pinkemell K. Scharalau NI, et al. Effect of freshly isolated autologous ticsue resident stromal cells on cardiac function and perfusion following acute myocardial infarction. tnt J Cardin?. 2009;141:26.35. 2. Lin K. Matsuhara, Masuda Y. et al. Ouractcrization of adipose tissue- derived cells isolated with Celotionm system. Cytotherapy. 2008:10: 4l7.426. EFTA R1_00377784 EFTA01929005